■ 基本信息
啟動子: | 無 |
復(fù)制子: | pVS1V |
終止子: | NOS |
質(zhì)粒分類: | 植物系列，蛋白過表達(dá)載體 |
質(zhì)粒大小: | 10549bp |
原核抗性: | Kan |
真核抗性: | Hyg |
克隆菌株: | DH5a |
培養(yǎng)條件: | 37度 |
表達(dá)宿主: | 植物細(xì)胞 |
5'測序引物: | 根據(jù)序列設(shè)計引物 |
3'測序引物: | 根據(jù)序列設(shè)計引物 |
■ 質(zhì)粒屬性
質(zhì)粒宿主: | 植物 |
質(zhì)粒用途: | 蛋白表達(dá) |
片段類型: |
|
片段物種: |
|
原核抗性: | Kan |
真核抗性: | Hyg
|
熒光標(biāo)記: | 綠色
|
■ 質(zhì)粒簡介
The pCambia1302 vector offers:1.High copy number in E.coli for high DNA yields2. pVS1 replicon for high stability in Agrobacterium 3.Small size4.Restriction sites designed for modular plasmid modifications and small but adequatepoly-linkers for introducing your DNA of interest5. Bacterial selection with kanamycin6.Plant selection with hygromycin B 7.Simple means to construct translational fusions to gusA reporter genes
質(zhì)粒只保證關(guān)鍵序列正確，不保證表達(dá)效果。
■ 質(zhì)粒圖譜
■ 質(zhì)粒序列
質(zhì)粒序列可參考（具體以測序?yàn)闇?zhǔn)）下載：
ZK109PCAMBIA1302.txt
