■ 基本信息
別名: | pCDNA3.1(-)-Myc-HisA |
啟動(dòng)子: | CMV |
復(fù)制子: | pUC |
終止子: | BGH poly（A）signal |
質(zhì)粒分類(lèi): | 哺乳細(xì)胞載體;蛋白過(guò)表達(dá)質(zhì)粒 |
質(zhì)粒大小: | 5522bp |
質(zhì)粒標(biāo)簽: | His Tag (6x), C-Myc Epitope Tag |
原核抗性: | Amp |
真核抗性: | G418 |
克隆菌株: | DH5a |
培養(yǎng)條件: | 37℃，有氧，LB |
表達(dá)宿主: | 哺乳細(xì)胞 |
5'測(cè)序引物: | T7 Fwd:5d[TAATACGACTCACTATAGGG]3 |
3'測(cè)序引物:
| 根據(jù)序列設(shè)計(jì)
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備注: |
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■ 質(zhì)粒屬性
質(zhì)粒宿主: | 哺乳細(xì)胞 |
質(zhì)粒用途: | 蛋白表達(dá) |
片段類(lèi)型: | ORF
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片段物種: | 空載體
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原核抗性: | Amp |
真核抗性: | G418
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熒光標(biāo)記: |
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■ 質(zhì)粒簡(jiǎn)介
pcDNA3.1/myc-His(-) A, B, and C are 5.5 kb vectors designed for overproduction of recombinant proteins in mammalian cell lines. The vectors are supplied in three reading frames to facilitate in frame cloning, with a C-terminal peptide containing a polyhistidine metal-binding tag and the myc (c-myc) epitope. The human cytomegalovirus immediate-early (CMV) promoter provides highlevel expression in a wide range of mammalian cells. In addition, the vector will replicate episomally in cell lines that are latently infected with SV40 or that express the SV40 large T antigen (e.g. COS7). High-level stable and nonreplicative transient expression can be carried out in most mammalian cells. The control plasmid, pcDNA3.1/myc-His(-)/lacZ, is the pcDNA3.1/myc-His(-) A vector with a 3.2 kb fragment containing the β-galactosidase gene cloned in frame with the C-terminal peptide. It is included for use as a positive control for transfection, expression, and detection in the cell line of choice.
質(zhì)粒只保證關(guān)鍵序列正確，不保證表達(dá)效果。
■ 質(zhì)粒圖譜
■ 質(zhì)粒序列
質(zhì)粒序列請(qǐng)下載：
ZK357pCDNA3.1-Myc-HisA(-)哺乳表達(dá)質(zhì)粒.txt
