■ 基本信息
別名: | 乳酸傳感 |
啟動(dòng)子: | CMV |
復(fù)制子: | pUC |
終止子: | SV40 poly(A) signal,bGH poly(A) signal |
質(zhì)粒分類: | 哺乳細(xì)胞，乳酸生物傳感器 |
質(zhì)粒大小: | 7648bp |
原核抗性: | Amp |
真核抗性: | G418 |
克隆菌株: | DH5a |
培養(yǎng)條件: | 37度 |
表達(dá)宿主: | 哺乳細(xì)胞 |
誘導(dǎo)方式: | 無須誘導(dǎo)，瞬時(shí)表達(dá) |
5'測(cè)序引物: | CMV-F：CGCAAATGGGCGGTAGGCGTG |
3'測(cè)序引物: | BGH:TAGAAGGCACAGTCGAGG |
■ 質(zhì)粒屬性
質(zhì)粒宿主: | 哺乳細(xì)胞 |
質(zhì)粒用途: | 蛋白表達(dá) |
片段類型: | ORF
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片段物種: |
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原核抗性: | Amp |
真核抗性: | G418
|
熒光標(biāo)記: |
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■ 質(zhì)粒簡(jiǎn)介
Lactate is shuttled between and inside cells, playing metabolic and signaling roles in healthy tissues. Lactate is also a harbinger of altered metabolism and participates in the pathogenesis of inflammation, hypoxia/ischemia, neurodegeneration and cancer. Many tumor cells show high rates of lactate production in the presence of oxygen, a phenomenon known as the Warburg effect, which has diagnostic and possibly therapeutic implications. In this article we introduce Laconic, a genetically-encoded Forster Resonance Energy Transfer (FRET)-based lactate sensor designed on the bacterial transcription factor LldR. Laconic quantified lactate from 1 μM to 10 mM and was not affected by glucose, pyruvate, acetate,
■ 質(zhì)粒圖譜
■ 質(zhì)粒序列
質(zhì)粒序列請(qǐng)下載：
ZK555pcDNA3.1-Laconic乳酸傳感質(zhì)粒.txt
